Abstract: Objective To observe the effect of emodin on nonalcoholic fatty liver disease ( NAFLD ) and the expression of 11β-HSD1 in liver. To investigate the expression changes of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in the liver of rats with nonalcoholic fatty liver ( NAFLD) and the intervention effect of emodin. Method 40 male Wistar rats were randomly divided into four groups: control, model, low-dosage emodin treatment ( E1) and high-dosage emodin treatment ( E2) groups. The control group was feed with normal diet, the model and treatment group were feed with high-fat diet. The treatment group was given emodin from the 9th week, and the experiment was completed at the end of the 14th week. Result Compared with the control group, rats in the model group had significant NAFLD, and the expression of 11β-HSD1 in liver was increased. Compared with the model group, emodin treatment could reduce liver index, blood lipid and liver lipid deposition, improve liver function and pathological changes, and significantly inhibit the expression of 11β-HSD1 gene and protein in liver tissue. All emodin treatment effects were dose-dependent. Conclusion Emodin has a significant protective effect on NAFLD, which maybe related to the inhibition of the 11β-HSD1 activity.

Key words: 11β-hydroxysteroid dehydrogenase type 1, emodin, nonalcoholic fatty liver disease, rat

摘要： 目的 观察大黄素对高热量饲料喂养所致大鼠非酒精性脂肪肝( NAFLD) 的治疗作用及对肝组织 11β-羟基类固醇脱氢酶 1(11β-hydroxysteroid dehydrogenase type 1,11β-HSDI)表达的影响。 方法 雄性 Wistar 大鼠 40 只,随机分为对照组、模型组、大黄素低剂量组( E1 组) 、大黄素高剂量组( E2 组) ,每组 10 只。 对照组给予普通饲料,其余 3 组喂以自制高脂饲料,从第 9 周开始治疗组给予大黄素干预治疗,第 14 周末实验结束,进行相关指标检测。结果 与对照组比较,模型组大鼠出现了明显的 NAFLD 病变,且肝组织 11β-HSD1 表达增加;与模型组比较,大黄素治疗可以降低肝脏指数,降低肝功能指标 ALT、AST,降低血脂及肝脏脂质沉积,减轻肝组织脂肪变性、炎性反应及纤维化,同时显著降低肝组织 11β-HSD1 基因和蛋白表达量。 且以上治疗作用呈剂量依赖性。 结论 大黄素对NAFLD 有保护作用,其作用机制可能与抑制 11β-HSD1 活性有关。

关键词: 11β-羟基类固醇脱氢酶 1, 大黄素, 非酒精性脂肪肝, 大鼠